Evidence for direct activation of mTORC2 kinase activity by phosphatidylinositol 3,4,5-trisphosphate

Gan X, Wang J, Su B, Wu D · 2011 · The Journal of biological chemistry · Atlas ID GAN2011

What this study shows

PIP3 directly stimulates mTORC2 kinase activity, linking PI3K to mTORC2.

Abstract

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mTORC2 (mammalian target of rapamycin complex 2) plays important roles in signal transduction by regulating an array of downstream effectors, including protein kinase AKT. However, its regulation by upstream regulators remains poorly characterized. Although phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P(3)) is known to regulate the phosphorylation of AKT Ser(473), the hydrophobic motif (HM) site, by mTORC2, it is not clear whether PtdIns(3,4,5)P(3) can directly regulate mTORC2 kinase activity.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: In vitro; cells) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	In vitro; cells
Journal	The Journal of biological chemistry
Year	2011
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1074/jbc.M110.195016 · PMID 21310961 · Free full text (PMC3064154)

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Biochemical (PtdIns(3,4,5)P3)
Target	mTORC2 / PIP3 / Akt Ser473
Model	In vitro; cells
Effect	PtdIns(3,4,5)P3 (PIP3) directly activates mTORC2 kinase activity toward Akt Ser473

In the Atlas

Links in the pathway map

Supports mTORC2 activates Akt/PKB
Supports PI3K activates mTORC2

Cite this paper

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Gan, X., Wang, J., Su, B., & Wu, D. (2011). Evidence for direct activation of mTORC2 kinase activity by phosphatidylinositol 3,4,5-trisphosphate. The Journal of biological chemistry. https://doi.org/10.1074/jbc.M110.195016

@article{GAN2011,
  author       = {Gan, X. and Wang, J. and Su, B. and Wu, D.},
  title        = {{Evidence for direct activation of mTORC2 kinase activity by phosphatidylinositol 3,4,5-trisphosphate}},
  journal      = {The Journal of biological chemistry},
  year         = {2011},
  doi          = {10.1074/jbc.M110.195016},
  note         = {PMID: 21310961},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record GAN2011) [Data set]. https://mtor-atlas.org/study/GAN2011/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_GAN2011,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record GAN2011},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/GAN2011/},
  doi          = {10.5281/zenodo.22059963}
}

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