Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis

Robitaille AM, Christen S, Shimobayashi M, Cornu M, Fava LL, Moes S, Prescianotto-Baschong C, Sauer U, Jenoe P, Hall MN · 2013 · Science · Atlas ID ROB2013

What this study shows

Quantitative phosphoproteomics show mTORC1 activates de novo pyrimidine synthesis.

Abstract

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The Ser-Thr kinase mammalian target of rapamycin (mTOR) controls cell growth and metabolism by stimulating glycolysis and synthesis of proteins and lipids. To further understand the central role of mTOR in cell physiology, we used quantitative phosphoproteomics to identify substrates or downstream effectors of the two mTOR complexes. mTOR controlled the phosphorylation of 335 proteins, including CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase). CAD catalyzes the first three steps in de novo pyrimidine synthesis.

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At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Mammalian cells) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Mammalian cells
Journal	Science
Year	2013
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1126/science.1228771 · PMID 23429704

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Quantitative phosphoproteomics
Target	mTORC1 / S6K1 / CAD
Model	Mammalian cells
Effect	mTORC1 activates de novo pyrimidine synthesis (CAD phosphorylation), among 335 mTOR-controlled phosphosites

In the Atlas

Links in the pathway map

Supports S6K1 activates Nucleotide synthesis

Cite this paper

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Robitaille, A. M., Christen, S., Shimobayashi, M., Cornu, M., Fava, L. L., Moes, S., Prescianotto-Baschong, C., Sauer, U., Jenoe, P., & Hall, M. N. (2013). Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science. https://doi.org/10.1126/science.1228771

@article{ROB2013,
  author       = {Robitaille, A. M. and Christen, S. and Shimobayashi, M. and Cornu, M. and Fava, L. L. and Moes, S. and Prescianotto-Baschong, C. and Sauer, U. and Jenoe, P. and Hall, M. N.},
  title        = {{Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis}},
  journal      = {Science},
  year         = {2013},
  doi          = {10.1126/science.1228771},
  note         = {PMID: 23429704},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record ROB2013) [Data set]. https://mtor-atlas.org/study/ROB2013/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_ROB2013,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record ROB2013},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/ROB2013/},
  doi          = {10.5281/zenodo.22059963}
}

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