Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis

Robitaille AM, Hall MN et al. · 2013 · Science · Atlas ID ROB2013

What this study shows

Quantitative phosphoproteomics show mTORC1 activates de novo pyrimidine synthesis.

Abstract

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The Ser-Thr kinase mammalian target of rapamycin (mTOR) controls cell growth and metabolism by stimulating glycolysis and synthesis of proteins and lipids. To further understand the central role of mTOR in cell physiology, we used quantitative phosphoproteomics to identify substrates or downstream effectors of the two mTOR complexes. mTOR controlled the phosphorylation of 335 proteins, including CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase). CAD catalyzes the first three steps in de novo pyrimidine synthesis.

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At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Mammalian cells) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Mammalian cells
Journal	Science
Year	2013
Peer reviewed	Yes
Record last updated	2026-08-22
Source	DOI 10.1126/science.1228771 · PMID 23429704

Extracted findings

Intervention	Quantitative phosphoproteomics
Target	mTORC1 / S6K1 / CAD
Model	Mammalian cells
Effect	mTORC1 activates de novo pyrimidine synthesis (CAD phosphorylation), among 335 mTOR-controlled phosphosites

In the Atlas

Cite this paper

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Robitaille, A. M., et al. (2013). Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science. https://doi.org/10.1126/science.1228771

@article{ROB2013,
  author       = {Robitaille, A. M. and Hall, M. N. and others},
  title        = {{Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis}},
  journal      = {Science},
  year         = {2013},
  doi          = {10.1126/science.1228771},
  note         = {PMID: 23429704},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record ROB2013) [Data set]. https://mtor-atlas.org/study/ROB2013/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_ROB2013,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record ROB2013},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/ROB2013/},
  doi          = {10.5281/zenodo.22059963}
}