De novo purine and pyrimidine production. mTORC1 matches supply to demand here, which is why proliferating cells are so dependent on it.
Making DNA and RNA building blocks.
S6K1 phosphorylates CAD for pyrimidines; ATF4–MTHFD2 supports purines. A growth output that is often forgotten next to translation.
| Tier | What it means | Studies |
|---|---|---|
| D | Mechanistic / in vitro / review | 4 |
No direct human evidence in the Atlas for this entity yet — everything below rests on animal or mechanistic work.
| Study | Year | Tier | Finding |
|---|---|---|---|
| VAL2017 | 2017 | D | Hyperactive mTORC1 couples nucleotide synthesis to demand; imbalance drives replication stress in these cells. |
| BEN2016 | 2016 | D | mTORC1 promotes purine synthesis via ATF4-driven mitochondrial tetrahydrofolate metabolism. |
| BEN2013 | 2013 | D | mTORC1-S6K1 stimulates de novo pyrimidine synthesis by phosphorylating CAD. |
| ROB2013 | 2013 | D | Quantitative phosphoproteomics show mTORC1 activates de novo pyrimidine synthesis. |