Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes

Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, Freinkman E, Sabatini DM · 2017 · Science · Atlas ID ABU2017

What this study shows

Turns the lysosome from a place where mTORC1 is switched on into something mTORC1 also controls: inhibiting mTOR strongly reduces the efflux of most essential amino acids out of the lysosome, so the organelle becomes a store rather than a source. Measured directly by rapidly isolating lysosomes and profiling their contents, which is why the claim is about concentrations inside the organelle and not inferred from signalling readouts. Boundary: one human cell line, acute pharmacological inhibition.

Abstract

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The lysosome degrades and recycles macromolecules, signals to the cytosol and nucleus, and is implicated in many diseases. Here, we describe a method for the rapid isolation of mammalian lysosomes and use it to quantitatively profile lysosomal metabolites under various cell states. Under nutrient-replete conditions, many lysosomal amino acids are in rapid exchange with those in the cytosol.

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At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Human cells (HEK293)) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Human cells (HEK293)
Journal	Science
Year	2017
Peer reviewed	Yes
Record last updated	2026-09-23
Source	DOI 10.1126/science.aan6298 · PMID 29074583

Cite this paper

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Abu-Remaileh, M., Wyant, G. A., Kim, C., Laqtom, N. N., Abbasi, M., Chan, S. H., Freinkman, E., & Sabatini, D. M. (2017). Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes. Science. https://doi.org/10.1126/science.aan6298

@article{ABU2017,
  author       = {Abu-Remaileh, M. and Wyant, G. A. and Kim, C. and Laqtom, N. N. and Abbasi, M. and Chan, S. H. and Freinkman, E. and Sabatini, D. M.},
  title        = {{Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes}},
  journal      = {Science},
  year         = {2017},
  doi          = {10.1126/science.aan6298},
  note         = {PMID: 29074583},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record ABU2017) [Data set]. https://mtor-atlas.org/study/ABU2017/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_ABU2017,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record ABU2017},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/ABU2017/},
  doi          = {10.5281/zenodo.22059963}
}