Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism

Gingras AC, Gygi SP, Raught B, Polakiewicz RD, Abraham RT, Hoekstra MF, Aebersold R, Sonenberg N · 1999 · Genes & development · Atlas ID GIN1999

What this study shows

4E-BP1 is phosphorylated by an ordered, priming two-step mechanism that controls its release of eIF4E.

Abstract

ShowHide

The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). Binding of the 4E-BPs to eIF4E is regulated by phosphorylation: Hypophosphorylated 4E-BP isoforms interact strongly with eIF4E, whereas hyperphosphorylated isoforms do not.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Mammalian cells; in vitro) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Mammalian cells; in vitro
Journal	Genes & development
Year	1999
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1101/gad.13.11.1422 · PMID 10364159 · Free full text (PMC316780)

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Biochemical (mTOR / 4E-BP1)
Target	mTOR / 4E-BP1 / eIF4E
Model	Mammalian cells; in vitro
Effect	4E-BP1 phosphorylation follows a two-step mechanism that regulates its binding to eIF4E

In the Atlas

Links in the pathway map

Supports mTORC1 inhibits 4E-BP1

Cite this paper

ShowHide

Gingras, A. C., Gygi, S. P., Raught, B., Polakiewicz, R. D., Abraham, R. T., Hoekstra, M. F., Aebersold, R., & Sonenberg, N. (1999). Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism. Genes & development. https://doi.org/10.1101/gad.13.11.1422

@article{GIN1999,
  author       = {Gingras, A. C. and Gygi, S. P. and Raught, B. and Polakiewicz, R. D. and Abraham, R. T. and Hoekstra, M. F. and Aebersold, R. and Sonenberg, N.},
  title        = {{Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism}},
  journal      = {Genes & development},
  year         = {1999},
  doi          = {10.1101/gad.13.11.1422},
  note         = {PMID: 10364159},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record GIN1999) [Data set]. https://mtor-atlas.org/study/GIN1999/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_GIN1999,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record GIN1999},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/GIN1999/},
  doi          = {10.5281/zenodo.22059963}
}

Was this page useful?