Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin

Brunn GJ, Hudson CC, Sekulić A, Williams JM, Hosoi H, Houghton PJ, Lawrence JC, Abraham RT · 1997 · Science · Atlas ID BRU1997

What this study shows

mTOR directly phosphorylates 4E-BP1 (PHAS-I), releasing eIF4E and establishing mTOR's role in translational control.

Abstract

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The immunosuppressant rapamycin interferes with G1-phase progression in lymphoid and other cell types by inhibiting the function of the mammalian target of rapamycin (mTOR). mTOR was determined to be a terminal kinase in a signaling pathway that couples mitogenic stimulation to the phosphorylation of the eukaryotic initiation factor (eIF)-4E-binding protein, PHAS-I. The rapamycin-sensitive protein kinase activity of mTOR was required for phosphorylation of PHAS-I in insulin-stimulated human embryonic kidney cells.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: HEK293; in vitro) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	HEK293; in vitro
Journal	Science
Year	1997
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1126/science.277.5322.99 · PMID 9204908

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Biochemical (mTOR/PHAS-I)
Target	mTOR / PHAS-I (4E-BP1) / eIF4E
Model	HEK293; in vitro
Effect	mTOR is a terminal kinase phosphorylating the translational repressor PHAS-I (4E-BP1)

In the Atlas

Links in the pathway map

Supports mTORC1 inhibits 4E-BP1
Supports Rapamycin inhibits mTORC1

Cite this paper

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Brunn, G. J., Hudson, C. C., Sekulić, A., Williams, J. M., Hosoi, H., Houghton, P. J., Lawrence, J. C., & Abraham, R. T. (1997). Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin. Science. https://doi.org/10.1126/science.277.5322.99

@article{BRU1997,
  author       = {Brunn, G. J. and Hudson, C. C. and Sekulić, A. and Williams, J. M. and Hosoi, H. and Houghton, P. J. and Lawrence, J. C. and Abraham, R. T.},
  title        = {{Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin}},
  journal      = {Science},
  year         = {1997},
  doi          = {10.1126/science.277.5322.99},
  note         = {PMID: 9204908},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record BRU1997) [Data set]. https://mtor-atlas.org/study/BRU1997/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_BRU1997,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record BRU1997},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/BRU1997/},
  doi          = {10.5281/zenodo.22059963}
}

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