Circadian regulation of mTOR by the ubiquitin pathway in renal cell carcinoma

Okazaki H, Matsunaga N, Fujioka T, Okazaki F, Akagawa Y, Tsurudome Y, Ono M, Kuwano M, Koyanagi S, Ohdo S · 2013 · Cancer Research · Atlas ID OKA2013

What this study shows

In mouse kidney tumours, active phosphorylated mTOR and total mTOR protein followed a 24-hour rhythm, set by the clock through the ubiquitin ligase Fbxw7. Giving everolimus when mTOR was high improved survival compared with other dosing times. Same drug, same dose - the clock time of dosing changed the outcome.

Abstract

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Circadian clock systems regulate many biologic functions, including cell division and hormone secretion in mammals. In this study, we explored the effects of circadian control on the pivot cell growth regulatory mTOR, the activity of which is deregulated in tumor cells compared with normal cells. Specifically, we investigated whether the antitumor effect of an mTOR inhibitor could be improved by changing its dosing schedule in RenCa tumor-bearing mice.

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At a glance

Evidence type	A Animal model Marked A because it is an animal intervention or observation study measuring an organismal outcome (model: RenCa tumour-bearing mice; NIH 3T3 cells); the code names the system studied -- animal work can be rigorous and still not be human data.
Study type	4 - Animal Study
Model system	RenCa tumour-bearing mice; NIH 3T3 cells
Journal	Cancer Research
Year	2013
Peer reviewed	Yes
Record last updated	2026-09-23
Source	DOI 10.1158/0008-5472.CAN-12-3241 · PMID 24253377

Extracted findings

Intervention	Everolimus dosed at different times of day
Target	mTOR; Fbxw7; DBP
Model	Mouse
Effect	Dosing everolimus at the mTOR peak improved survival of tumour-bearing mice

In the Atlas

Cite this paper

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Okazaki, H., Matsunaga, N., Fujioka, T., Okazaki, F., Akagawa, Y., Tsurudome, Y., Ono, M., Kuwano, M., Koyanagi, S., & Ohdo, S. (2013). Circadian regulation of mTOR by the ubiquitin pathway in renal cell carcinoma. Cancer Research. https://doi.org/10.1158/0008-5472.CAN-12-3241

@article{OKA2013,
  author       = {Okazaki, H. and Matsunaga, N. and Fujioka, T. and Okazaki, F. and Akagawa, Y. and Tsurudome, Y. and Ono, M. and Kuwano, M. and Koyanagi, S. and Ohdo, S.},
  title        = {{Circadian regulation of mTOR by the ubiquitin pathway in renal cell carcinoma}},
  journal      = {Cancer Research},
  year         = {2013},
  doi          = {10.1158/0008-5472.CAN-12-3241},
  note         = {PMID: 24253377},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record OKA2013) [Data set]. https://mtor-atlas.org/study/OKA2013/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_OKA2013,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record OKA2013},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/OKA2013/},
  doi          = {10.5281/zenodo.22059963}
}