SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity

Jacinto E, Facchinetti V, Liu D, Soto N, Wei S, Jung SY, Huang Q, Qin J, Su B · 2006 · Cell · Atlas ID JAC2006

What this study shows

SIN1 maintains rictor-mTOR integrity and confers mTORC2 Akt-Ser473 kinase activity and substrate specificity.

Abstract

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Mammalian target of rapamycin (mTOR) controls cell growth and proliferation via the raptor-mTOR (TORC1) and rictor-mTOR (TORC2) protein complexes. Recent biochemical studies suggested that TORC2 is the elusive PDK2 for Akt/PKB Ser473 phosphorylation in the hydrophobic motif. Phosphorylation at Ser473, along with Thr308 of its activation loop, is deemed necessary for Akt function, although the regulatory mechanisms and physiological importance of each phosphorylation site remain to be fully understood. Here, we report that SIN1/MIP1 is an essential TORC2/PDK2 subunit.

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At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Mammalian cells) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Mammalian cells
Journal	Cell
Year	2006
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1016/j.cell.2006.08.033 · PMID 16962653

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Biochemical/genetic (SIN1/MIP1)
Target	mTORC2 (rictor-mTOR) / Akt Ser473
Model	Mammalian cells
Effect	SIN1 maintains rictor–mTOR (mTORC2) integrity and controls Akt Ser473 phosphorylation and substrate specificity

In the Atlas

Links in the pathway map

Supports mTORC2 activates Akt/PKB
Supports SIN1 / MAPKAP1 required-for mTORC2
Supports Akt/PKB inhibits FOXO1/3

Learn the biology

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Cite this paper

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Jacinto, E., Facchinetti, V., Liu, D., Soto, N., Wei, S., Jung, S. Y., Huang, Q., Qin, J., & Su, B. (2006). SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell. https://doi.org/10.1016/j.cell.2006.08.033

@article{JAC2006,
  author       = {Jacinto, E. and Facchinetti, V. and Liu, D. and Soto, N. and Wei, S. and Jung, S. Y. and Huang, Q. and Qin, J. and Su, B.},
  title        = {{SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity}},
  journal      = {Cell},
  year         = {2006},
  doi          = {10.1016/j.cell.2006.08.033},
  note         = {PMID: 16962653},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record JAC2006) [Data set]. https://mtor-atlas.org/study/JAC2006/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_JAC2006,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record JAC2006},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/JAC2006/},
  doi          = {10.5281/zenodo.22059963}
}

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