mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s

Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM · 2006 · Current biology : CB · Atlas ID FRI2006

What this study shows

mSin1 is required for mTORC2 assembly and Akt Ser473 phosphorylation; isoforms define distinct complexes.

Abstract

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The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that participates in at least two distinct multiprotein complexes, mTORC1 and mTORC2 . These complexes play important roles in the regulation of cell growth, proliferation, survival, and metabolism. mTORC2 is a hydrophobic motif kinase for the cell-survival protein Akt/PKB and, here, we identify mSin1 as a component of mTORC2 but not mTORC1. mSin1 is necessary for the assembly of mTORC2 and for its capacity to phosphorylate Akt/PKB.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Mammalian cells) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Mammalian cells
Journal	Current biology : CB
Year	2006
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1016/j.cub.2006.08.001 · PMID 16919458

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Biochemical/genetic (mSin1)
Target	mTORC2 / mSin1 / Akt
Model	Mammalian cells
Effect	mSin1 is required for Akt/PKB phosphorylation; its isoforms define three distinct mTORC2 complexes

In the Atlas

Links in the pathway map

Supports mTORC2 activates Akt/PKB
Supports SIN1 / MAPKAP1 required-for mTORC2

Cite this paper

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Frias, M. A., Thoreen, C. C., Jaffe, J. D., Schroder, W., Sculley, T., Carr, S. A., & Sabatini, D. M. (2006). mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s. Current biology : CB. https://doi.org/10.1016/j.cub.2006.08.001

@article{FRI2006,
  author       = {Frias, M. A. and Thoreen, C. C. and Jaffe, J. D. and Schroder, W. and Sculley, T. and Carr, S. A. and Sabatini, D. M.},
  title        = {{mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s}},
  journal      = {Current biology : CB},
  year         = {2006},
  doi          = {10.1016/j.cub.2006.08.001},
  note         = {PMID: 16919458},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record FRI2006) [Data set]. https://mtor-atlas.org/study/FRI2006/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_FRI2006,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record FRI2006},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/FRI2006/},
  doi          = {10.5281/zenodo.22059963}
}

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