Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes

Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM · 2018 · Nature · Atlas ID SHE2018

What this study shows

Cryo-EM structures of GATOR1 and GATOR1-Rag complexes reveal GAP and inhibitory-clamp mechanisms.

Abstract

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Nutrients, such as amino acids and glucose, signal through the Rag GTPases to activate mTORC1. The GATOR1 protein complex-comprising DEPDC5, NPRL2 and NPRL3-regulates the Rag GTPases as a GTPase-activating protein (GAP) for RAGA; loss of GATOR1 desensitizes mTORC1 signalling to nutrient starvation. GATOR1 components have no sequence homology to other proteins, so the function of GATOR1 at the molecular level is currently unknown. Here we used cryo-electron microscopy to solve structures of GATOR1 and GATOR1-Rag GTPases complexes.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Cryo-EM structure) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Cryo-EM structure
Journal	Nature
Year	2018
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1038/nature26158 · PMID 29590090 · Free full text (PMC5975964)

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Structural (cryo-EM)
Target	GATOR1 (DEPDC5/NPRL2/NPRL3) / RagA / mTORC1
Model	Cryo-EM structure
Effect	Cryo-EM structures of GATOR1 and GATOR1-Rag reveal its GAP activity and inhibitory-clamp mechanism

In the Atlas

Links in the pathway map

Supports GATOR1 inhibits Rag GTPases

Cite this paper

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Shen, K., Huang, R. K., Brignole, E. J., Condon, K. J., Valenstein, M. L., Chantranupong, L., Bomaliyamu, A., Choe, A., Hong, C., Yu, Z., & Sabatini, D. M. (2018). Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes. Nature. https://doi.org/10.1038/nature26158

@article{SHE2018,
  author       = {Shen, K. and Huang, R. K. and Brignole, E. J. and Condon, K. J. and Valenstein, M. L. and Chantranupong, L. and Bomaliyamu, A. and Choe, A. and Hong, C. and Yu, Z. and Sabatini, D. M.},
  title        = {{Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes}},
  journal      = {Nature},
  year         = {2018},
  doi          = {10.1038/nature26158},
  note         = {PMID: 29590090},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record SHE2018) [Data set]. https://mtor-atlas.org/study/SHE2018/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_SHE2018,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record SHE2018},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/SHE2018/},
  doi          = {10.5281/zenodo.22059963}
}

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