Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism

Hara K, Yonezawa K, Weng QP, Kozlowski MT, Belham C, Avruch J · 1998 · The Journal of biological chemistry · Atlas ID HAR1998

What this study shows

Amino acid sufficiency signals through mTOR to p70 S6K and 4E-BP1 via a common effector, first linking nutrients to mTOR.

Abstract

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The present study identifies the operation of a signal tranduction pathway in mammalian cells that provides a checkpoint control, linking amino acid sufficiency to the control of peptide chain initiation. Withdrawal of amino acids from the nutrient medium of CHO-IR cells results in a rapid deactivation of p70 S6 kinase and dephosphorylation of eIF-4E BP1, which become unresponsive to all agonists. Readdition of the amino acid mixture quickly restores the phosphorylation and responsiveness of p70 and eIF-4E BP1 to insulin.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: CHO cells; in vitro) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	CHO cells; in vitro
Journal	The Journal of biological chemistry
Year	1998
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1074/jbc.273.23.14484 · PMID 9603962

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Biochemical (amino-acid withdrawal)
Target	mTOR / p70 S6K / 4E-BP1
Model	CHO cells; in vitro
Effect	Amino-acid sufficiency and mTOR regulate p70 S6K and 4E-BP1 through a common effector mechanism

In the Atlas

Links in the pathway map

Supports mTORC1 inhibits 4E-BP1
Supports mTORC1 activates S6K1

Learn the biology

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Cite this paper

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Hara, K., Yonezawa, K., Weng, Q. P., Kozlowski, M. T., Belham, C., & Avruch, J. (1998). Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism. The Journal of biological chemistry. https://doi.org/10.1074/jbc.273.23.14484

@article{HAR1998,
  author       = {Hara, K. and Yonezawa, K. and Weng, Q. P. and Kozlowski, M. T. and Belham, C. and Avruch, J.},
  title        = {{Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism}},
  journal      = {The Journal of biological chemistry},
  year         = {1998},
  doi          = {10.1074/jbc.273.23.14484},
  note         = {PMID: 9603962},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record HAR1998) [Data set]. https://mtor-atlas.org/study/HAR1998/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_HAR1998,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record HAR1998},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/HAR1998/},
  doi          = {10.5281/zenodo.22059963}
}

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