Oliver's mTOR Atlas Evidence Platform
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Fluorescent protein ticker tape (FPTT): Multiplexed recording of transcriptional dynamics in living cells and in vivo

Wang R, Jiang J et al. · 2026 · Science Advances · Atlas ID WANG2026C

What this study shows

Engineered a multiplexed fluorescent-protein 'ticker tape' biosensor platform (self-assembling protein fibers + multispectral fluorescent proteins) for longitudinal, single-cell recording of signaling-pathway transcriptional histories (mTOR, NF-κB, STAT3, NFAT, cAMP). Applying the mTOR-FPTT reporter, the authors independently observed cell-cycle-dependent OSCILLATING mTOR activity dynamics -- a new, orthogonal tool corroborating that mTOR signaling is patterned over time rather than static, consistent with JOS2024's cell-cycle oscillation finding.

Abstract

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Recording and real-time imaging of promoter activities are critical for deciphering signaling cross-talk, but technologies for simultaneously capturing multiple transient events in living cells are lacking. Here, we design fluorescent protein-based ticker tapes (FPTT) for multiplexed, scalable, longitudinal recording of single-cell physiological activities by integrating multispectral monomeric fluorescent proteins with self-assembling protein fibers. FPTT logged dose-dependent, reversible endogenous cFos transcriptional histories in hippocampal neurons at 3-hour resolution over 8 days.

Read the full abstract on PubMed →

At a glance

Evidence type M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Human & mouse cell lines (hippocampal neurons, T cells); mouse liver in vivo — live single-cell longitudinal imaging) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type5 - Mechanistic / In Vitro
Model systemHuman & mouse cell lines (hippocampal neurons, T cells); mouse liver in vivo — live single-cell longitudinal imaging
JournalScience Advances
Year2026
Peer reviewedYes
Record last updated2026-08-22
SourceDOI 10.1126/sciadv.aef9406 · PMID 42685187 · Free full text (PMC13537258)

In the Atlas

Related topics

mTORmTORC1

Cite this paper

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Wang, R., et al. (2026). Fluorescent protein ticker tape (FPTT): Multiplexed recording of transcriptional dynamics in living cells and in vivo. Science Advances. https://doi.org/10.1126/sciadv.aef9406

@article{WANG2026C,
  author       = {Wang, R. and Jiang, J. and others},
  title        = {{Fluorescent protein ticker tape (FPTT): Multiplexed recording of transcriptional dynamics in living cells and in vivo}},
  journal      = {Science Advances},
  year         = {2026},
  doi          = {10.1126/sciadv.aef9406},
  note         = {PMID: 42685187},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper. 

Barton, O. (2026). Oliver's mTOR Atlas (record WANG2026C) [Data set]. https://mtor-atlas.org/study/WANG2026C/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_WANG2026C,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record WANG2026C},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/WANG2026C/},
  doi          = {10.5281/zenodo.22059963}
}