hVps34 is a nutrient-regulated lipid kinase required for activation of p70 S6 kinase
What this study shows
Opens a second, non-canonical route by which amino acids reach mTORC1. The class III PI 3-kinase hVps34 is switched off by amino acid or glucose starvation and by AMPK activation, and is required for S6K1 and 4E-BP1 phosphorylation, yet it sits outside the insulin/class I PI3K input (it does not affect Akt or TSC2 phosphorylation). Boundary: cell lines and knockdown/overexpression only; the step is nutrient-regulated but the sensor itself is not identified.
Abstract
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Mammalian cells respond to nutrient deprivation by inhibiting energy consuming processes, such as proliferation and protein synthesis, and by stimulating catabolic processes, such as autophagy. p70 S6 kinase (S6K1) plays a central role during nutritional regulation of translation. S6K1 is activated by growth factors such as insulin, and by mammalian target of rapamycin (mTOR), which is itself regulated by amino acids. The Class IA phosphatidylinositol (PI) 3-kinase plays a well recognized role in the regulation of S6K1.
Read the full abstract on PubMed →At a glance
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| Evidence type | M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Human and hamster cell lines (HeLa, CHO)) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is. |
| Study type | 5 - Mechanistic / In Vitro |
| Model system | Human and hamster cell lines (HeLa, CHO) |
| Journal | Journal of Biological Chemistry |
| Year | 2005 |
| Peer reviewed | Yes |
| Record last updated | 2026-10-01 |
| Source | DOI 10.1074/jbc.M507201200 · PMID 16049009 |
Where this study fits
How this study connects to the pathway map, open questions, people and other studies in the Atlas.
People in the Atlas
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Showed as first author that the lipid kinase hVps34 is regulated by nutrients and is needed for amino acids to activate S6 kinase through mTOR, in parallel with a study from George Thomas's group
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Showed that the class III PI 3-kinase hVps34, best known for its role in autophagy, is regulated by nutrients and is needed for amino acids to activate S6 kinase through mTOR, in parallel with George Thomas's group
More: 1 other author
No profile yet: Murray JT
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Topics
Extracted findings
These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.
| Intervention | hVps34 overexpression, siRNA knockdown and inhibitory antibody microinjection; amino-acid/glucose starvation; AMPK activation |
| Target | hVps34 -> mTOR/S6K1 (nutrient-regulated input) |
| Model | Human and hamster cell lines (HeLa, CHO) |
| Effect | hVps34 is inhibited by nutrient starvation and AMPK and is required for S6K1 and 4E-BP1 phosphorylation, acting as a nutrient-regulated input to mTOR independent of insulin/class I PI3K-Akt. |
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