Rapamycin-induced inhibition of the 70-kilodalton S6 protein kinase

Price DJ, Grove JR, Calvo V, Avruch J, Bierer BE · 1992 · Science · Atlas ID PRI1992

What this study shows

Rapamycin induces dephosphorylation/inactivation of the 70 kDa S6 kinase, defining an early readout of TOR signalling.

Abstract

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The immunosuppressant rapamycin inhibited proliferation of the H4IIEC hepatoma cell line. Rapamycin, but not its structural analog FK506, also inhibited the basal and insulin-stimulated activity of the p70 ribosomal protein S6 kinase. By contrast, insulin stimulation of the p85 Rsk S6 kinase and mitogen-activated protein (MAP) kinase activity were unaffected by drug.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Mammalian cells; in vitro) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Mammalian cells; in vitro
Journal	Science
Year	1992
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1126/science.1380182 · PMID 1380182

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Rapamycin (vs FK506)
Target	p70 S6 kinase
Model	Mammalian cells; in vitro
Effect	Rapamycin (not FK506) inhibits basal and insulin-stimulated p70 S6 kinase activity

In the Atlas

Answers that reference this study

Discussed in the plain-language answer What is mTOR?.

Learn the biology

Want to understand the biology behind this study? → Challenge 02: Why rapamycin does not switch mTORC1 off

Cite this paper

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Price, D. J., Grove, J. R., Calvo, V., Avruch, J., & Bierer, B. E. (1992). Rapamycin-induced inhibition of the 70-kilodalton S6 protein kinase. Science. https://doi.org/10.1126/science.1380182

@article{PRI1992,
  author       = {Price, D. J. and Grove, J. R. and Calvo, V. and Avruch, J. and Bierer, B. E.},
  title        = {{Rapamycin-induced inhibition of the 70-kilodalton S6 protein kinase}},
  journal      = {Science},
  year         = {1992},
  doi          = {10.1126/science.1380182},
  note         = {PMID: 1380182},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record PRI1992) [Data set]. https://mtor-atlas.org/study/PRI1992/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_PRI1992,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record PRI1992},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/PRI1992/},
  doi          = {10.5281/zenodo.22059963}
}

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