The proline-rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1

The proline-rich Akt substrate of 40 kilodaltons (PRAS40) was identified as a raptor-binding protein that is phosphorylated directly by mammalian target of rapamycin (mTOR) complex 1 (mTORC1) but not mTORC2 in vitro, predominantly at PRAS40 (Ser(183)). The binding of S6K1 and 4E-BP1 to raptor requires a TOR signaling (TOS) motif, which contains an essential Phe followed by four alternating acidic and small hydrophobic amino acids. PRAS40 binding to raptor was severely inhibited by mutation of PRAS40 (Phe(129) to Ala).

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Oshiro, N., et al. (2007). The proline-rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1. The Journal of biological chemistry. https://doi.org/10.1074/jbc.M702636200

@article{OSH2007,
  author       = {Oshiro, N. and Yonezawa, K. and others},
  title        = {{The proline-rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1}},
  journal      = {The Journal of biological chemistry},
  year         = {2007},
  doi          = {10.1074/jbc.M702636200},
  note         = {PMID: 17517883},
}

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The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record OSH2007) [Data set]. https://mtor-atlas.org/study/OSH2007/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_OSH2007,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record OSH2007},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/OSH2007/},
  doi          = {10.5281/zenodo.22059963}
}