The Sestrins interact with GATOR2 to negatively regulate the amino-acid-sensing pathway upstream of mTORC1

Chantranupong L, Sabatini DM et al. · 2014 · Cell reports · Atlas ID CHA2014

What this study shows

Sestrins bind GATOR2 to negatively regulate amino-acid signalling upstream of mTORC1.

Abstract

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The mechanistic target of rapamycin complex 1 (mTORC1) kinase is a major regulator of cell growth that responds to numerous environmental cues. A key input is amino acids, which act through the heterodimeric Rag GTPases (RagA or RagB bound to RagC or RagD) in order to promote the translocation of mTORC1 to the lysosomal surface, its site of activation. GATOR2 is a complex of unknown function that positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway.

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At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Mammalian cells) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Mammalian cells
Journal	Cell reports
Year	2014
Peer reviewed	Yes
Record last updated	2026-08-22
Source	DOI 10.1016/j.celrep.2014.09.014 · PMID 25263562 · Free full text (PMC4223866)

Extracted findings

Intervention	Biochemical/genetic (Sestrins)
Target	Sestrins / GATOR2 / Rag / mTORC1
Model	Mammalian cells
Effect	Sestrins bind GATOR2 to negatively regulate the amino-acid-sensing pathway upstream of mTORC1

Cite this paper

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Chantranupong, L., et al. (2014). The Sestrins interact with GATOR2 to negatively regulate the amino-acid-sensing pathway upstream of mTORC1. Cell reports. https://doi.org/10.1016/j.celrep.2014.09.014

@article{CHA2014,
  author       = {Chantranupong, L. and Sabatini, D. M. and others},
  title        = {{The Sestrins interact with GATOR2 to negatively regulate the amino-acid-sensing pathway upstream of mTORC1}},
  journal      = {Cell reports},
  year         = {2014},
  doi          = {10.1016/j.celrep.2014.09.014},
  note         = {PMID: 25263562},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record CHA2014) [Data set]. https://mtor-atlas.org/study/CHA2014/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_CHA2014,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record CHA2014},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/CHA2014/},
  doi          = {10.5281/zenodo.22059963}
}