Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling

Yu Yonghao, Yoon SO, Poulogiannis G, Yang Q, Ma XM, Villén J, Kubica N, Hoffman GR, Cantley LC, Gygi SP, Blenis J · 2011 · Science · Atlas ID YUX2011

What this study shows

Phosphoproteomics identify Grb10 as an mTORC1 substrate driving negative feedback on insulin/PI3K.

Abstract

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The evolutionarily conserved serine-threonine kinase mammalian target of rapamycin (mTOR) plays a critical role in regulating many pathophysiological processes. Functional characterization of the mTOR signaling pathways, however, has been hampered by the paucity of known substrates. We used large-scale quantitative phosphoproteomics experiments to define the signaling networks downstream of mTORC1 and mTORC2.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Mammalian cells) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Mammalian cells
Journal	Science
Year	2011
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1126/science.1199484 · PMID 21659605 · Free full text (PMC3195509)

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Phosphoproteomics (mTORC1/Grb10)
Target	mTORC1 / Grb10 / insulin signaling
Model	Mammalian cells
Effect	Grb10 is an mTORC1 substrate that negatively feeds back on insulin/IGF signaling

In the Atlas

Links in the pathway map

Supports Grb10 inhibits Growth hormone / IGF-1 axis
Supports mTORC1 activates Grb10

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Cite this paper

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Yu Yonghao, Yoon, S. O., Poulogiannis, G., Yang, Q., Ma, X. M., Villén, J., Kubica, N., Hoffman, G. R., Cantley, L. C., Gygi, S. P., & Blenis, J. (2011). Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science. https://doi.org/10.1126/science.1199484

@article{YUX2011,
  author       = {Yu Yonghao and Yoon, S. O. and Poulogiannis, G. and Yang, Q. and Ma, X. M. and Villén, J. and Kubica, N. and Hoffman, G. R. and Cantley, L. C. and Gygi, S. P. and Blenis, J.},
  title        = {{Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling}},
  journal      = {Science},
  year         = {2011},
  doi          = {10.1126/science.1199484},
  note         = {PMID: 21659605},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record YUX2011) [Data set]. https://mtor-atlas.org/study/YUX2011/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_YUX2011,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record YUX2011},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/YUX2011/},
  doi          = {10.5281/zenodo.22059963}
}

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