Cryo-EM Structure of the Human FLCN-FNIP2-Rag-Ragulator Complex

Shen K, Rogala KB, Chou HT, Huang RK, Yu Z, Sabatini DM · 2019 · Cell · Atlas ID SHE2019

What this study shows

Cryo-EM structure of the human FLCN-FNIP2-Rag-Ragulator complex.

Abstract

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mTORC1 controls anabolic and catabolic processes in response to nutrients through the Rag GTPase heterodimer, which is regulated by multiple upstream protein complexes. One such regulator, FLCN-FNIP2, is a GTPase activating protein (GAP) for RagC/D, but despite its important role, how it activates the Rag GTPase heterodimer remains unknown. We used cryo-EM to determine the structure of FLCN-FNIP2 in a complex with the Rag GTPases and Ragulator. FLCN-FNIP2 adopts an extended conformation with two pairs of heterodimerized domains.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Cryo-EM structure) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Cryo-EM structure
Journal	Cell
Year	2019
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1016/j.cell.2019.10.036 · PMID 31704029 · Free full text (PMC7008705)

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Structural (cryo-EM)
Target	FLCN-FNIP2 / Rag / Ragulator / mTORC1
Model	Cryo-EM structure
Effect	Cryo-EM structure reveals how FLCN-FNIP2 engages the Rag GTPase heterodimer and Ragulator

In the Atlas

Links in the pathway map

Supports FLCN / FNIP1/2 activates Rag GTPases

Cite this paper

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Shen, K., Rogala, K. B., Chou, H. T., Huang, R. K., Yu, Z., & Sabatini, D. M. (2019). Cryo-EM Structure of the Human FLCN-FNIP2-Rag-Ragulator Complex. Cell. https://doi.org/10.1016/j.cell.2019.10.036

@article{SHE2019,
  author       = {Shen, K. and Rogala, K. B. and Chou, H. T. and Huang, R. K. and Yu, Z. and Sabatini, D. M.},
  title        = {{Cryo-EM Structure of the Human FLCN-FNIP2-Rag-Ragulator Complex}},
  journal      = {Cell},
  year         = {2019},
  doi          = {10.1016/j.cell.2019.10.036},
  note         = {PMID: 31704029},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record SHE2019) [Data set]. https://mtor-atlas.org/study/SHE2019/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_SHE2019,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record SHE2019},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/SHE2019/},
  doi          = {10.5281/zenodo.22059963}
}

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