Oliver's mTOR Atlas Evidence Platform
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Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex

Sarbassov DD et al. · 2005 · Science · Atlas ID SAR2005

What this study shows

The rictor-mTOR complex (mTORC2) directly phosphorylates Akt/PKB on Ser473, regulating cell survival.

Abstract

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Deregulation of Akt/protein kinase B (PKB) is implicated in the pathogenesis of cancer and diabetes. Akt/PKB activation requires the phosphorylation of Thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (PDK1) and Ser473 within the carboxyl-terminal hydrophobic motif by an unknown kinase. We show that in Drosophila and human cells the target of rapamycin (TOR) kinase and its associated protein rictor are necessary for Ser473 phosphorylation and that a reduction in rictor or mammalian TOR (mTOR) expression inhibited an Akt/PKB effector.

Read the full abstract on PubMed →

At a glance

Evidence type M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Human cells; Drosophila) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type5 - Mechanistic / In Vitro
Model systemHuman cells; Drosophila
JournalScience
Year2005
Peer reviewedYes
Record last updated2026-08-22
SourceDOI 10.1126/science.1106148 · PMID 15718470

Extracted findings

InterventionGenetic/biochemical (rictor-mTOR)
TargetmTORC2 (rictor) / Akt Ser473
ModelHuman cells; Drosophila
EffectThe rictor-mTOR complex (mTORC2) phosphorylates Akt/PKB at Ser473

In the Atlas

Related topics

Akt/PKBmTORC2

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Cite this paper

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Sarbassov, D. D., et al. (2005). Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. https://doi.org/10.1126/science.1106148

@article{SAR2005,
  author       = {Sarbassov, D. D. and others},
  title        = {{Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex}},
  journal      = {Science},
  year         = {2005},
  doi          = {10.1126/science.1106148},
  note         = {PMID: 15718470},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper. 

Barton, O. (2026). Oliver's mTOR Atlas (record SAR2005) [Data set]. https://mtor-atlas.org/study/SAR2005/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_SAR2005,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record SAR2005},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/SAR2005/},
  doi          = {10.5281/zenodo.22059963}
}