mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events

Holz MK, Blenis J et al. · 2005 · Cell · Atlas ID HOL2005

What this study shows

mTOR and S6K1 dynamically assemble the translation preinitiation complex on eIF3.

Abstract

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In response to nutrients, energy sufficiency, hormones, and mitogenic agents, S6K1 phosphorylates several targets linked to translation. However, the molecular mechanisms whereby S6K1 is activated, encounters substrate, and contributes to translation initiation are poorly understood. We show that mTOR and S6K1 maneuver on and off the eukaryotic initiation factor 3 (eIF3) translation initiation complex in a signal-dependent, choreographed fashion. When inactive, S6K1 associates with the eIF3 complex, while the S6K1 activator mTOR/raptor does not.

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At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Mammalian cells) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Mammalian cells
Journal	Cell
Year	2005
Peer reviewed	Yes
Record last updated	2026-08-22
Source	DOI 10.1016/j.cell.2005.10.024 · PMID 16286006

Extracted findings

Intervention	Biochemical
Target	mTOR / S6K1 / eIF3
Model	Mammalian cells
Effect	mTOR and S6K1 dynamically associate with eIF3 to control translation preinitiation via ordered phosphorylation

Cite this paper

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Holz, M. K., et al. (2005). mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events. Cell. https://doi.org/10.1016/j.cell.2005.10.024

@article{HOL2005,
  author       = {Holz, M. K. and Blenis, J. and others},
  title        = {{mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events}},
  journal      = {Cell},
  year         = {2005},
  doi          = {10.1016/j.cell.2005.10.024},
  note         = {PMID: 16286006},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record HOL2005) [Data set]. https://mtor-atlas.org/study/HOL2005/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_HOL2005,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record HOL2005},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/HOL2005/},
  doi          = {10.5281/zenodo.22059963}
}