Oliver's mTOR Atlas Evidence Platform
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Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction

Condon KJ, Orozco JM, Adelmann CH, Spinelli JB, van der Helm PW, Roberts JM, Kunchok T, Sabatini DM · 2021 · Proceedings of the National Academy of Sciences of the United States of America · Atlas ID CON2021

What this study shows

Genome-wide CRISPR screens reveal AMPK and HRI relay mitochondrial dysfunction to mTORC1.

Abstract

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In mammalian cells, nutrients and growth factors signal through an array of upstream proteins to regulate the mTORC1 growth control pathway. Because the full complement of these proteins has not been systematically identified, we developed a FACS-based CRISPR-Cas9 genetic screening strategy to pinpoint genes that regulate mTORC1 activity. Along with almost all known positive components of the mTORC1 pathway, we identified many genes that impact mTORC1 activity.

Read the full abstract on PubMed →

At a glance

Evidence type M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: CRISPR screen; cells) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type5 - Mechanistic / In Vitro
Model systemCRISPR screen; cells
JournalProceedings of the National Academy of Sciences of the United States of America
Year2021
Peer reviewedYes
Record last updated2026-10-01
SourceDOI 10.1073/pnas.2022120118 · PMID 33483422 · Free full text (PMC7848693)

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

InterventionGenome-wide CRISPR-Cas9 screen
TargetmTORC1 (mitochondrial-dysfunction sensing)
ModelCRISPR screen; cells
EffectCRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction

In the Atlas

Related topics

Mitochondrial dysfunction

Links in the pathway map

Cite this paper

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Condon, K. J., Orozco, J. M., Adelmann, C. H., Spinelli, J. B., van der Helm, P. W., Roberts, J. M., Kunchok, T., & Sabatini, D. M. (2021). Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.2022120118

@article{CON2021,
  author       = {Condon, K. J. and Orozco, J. M. and Adelmann, C. H. and Spinelli, J. B. and van der Helm, P. W. and Roberts, J. M. and Kunchok, T. and Sabatini, D. M.},
  title        = {{Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction}},
  journal      = {Proceedings of the National Academy of Sciences of the United States of America},
  year         = {2021},
  doi          = {10.1073/pnas.2022120118},
  note         = {PMID: 33483422},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper. 

Barton, O. (2026). Oliver's mTOR Atlas (record CON2021) [Data set]. https://mtor-atlas.org/study/CON2021/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_CON2021,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record CON2021},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/CON2021/},
  doi          = {10.5281/zenodo.22059963}
}
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