Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction

In mammalian cells, nutrients and growth factors signal through an array of upstream proteins to regulate the mTORC1 growth control pathway. Because the full complement of these proteins has not been systematically identified, we developed a FACS-based CRISPR-Cas9 genetic screening strategy to pinpoint genes that regulate mTORC1 activity. Along with almost all known positive components of the mTORC1 pathway, we identified many genes that impact mTORC1 activity.

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Condon, K. J., et al. (2021). Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.2022120118

@article{CON2021,
  author       = {Condon, K. J. and Sabatini, D. M. and others},
  title        = {{Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction}},
  journal      = {Proceedings of the National Academy of Sciences of the United States of America},
  year         = {2021},
  doi          = {10.1073/pnas.2022120118},
  note         = {PMID: 33483422},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record CON2021) [Data set]. https://mtor-atlas.org/study/CON2021/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_CON2021,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record CON2021},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/CON2021/},
  doi          = {10.5281/zenodo.22059963}
}