Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells

Sabers CJ, Martin MM, Brunn GJ, Williams JM, Dumont FJ, Wiederrecht G, Abraham RT · 1995 · The Journal of biological chemistry · Atlas ID SAB1995

What this study shows

Isolated mTOR as the FKBP12-rapamycin-associated protein controlling G1-S progression.

Abstract

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The immunosuppressive drug, rapamycin, interferes with an undefined signaling pathway required for the progression of G1-phase T-cells into S phase. Genetic analyses in yeast indicate that binding of rapamycin to its intracellular receptor, FKBP12, generates a toxic complex that inhibits cell growth in G1 phase. These analyses implicated two related proteins, TOR1 and TOR2, as targets of the FKBP12-rapamycin complex in yeast.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: Rat/mouse cells; in vitro) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	Rat/mouse cells; in vitro
Journal	The Journal of biological chemistry
Year	1995
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1074/jbc.270.2.815 · PMID 7822316

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Biochemical (FKBP12-rapamycin affinity)
Target	mTOR (FRAP/RAFT1) / FKBP12
Model	Rat/mouse cells; in vitro
Effect	Isolation of the mammalian FKBP12-rapamycin target protein (mTOR)

In the Atlas

Links in the pathway map

Learn the biology

Want to understand the biology behind this study? → Challenge 02: Why rapamycin does not switch mTORC1 off

Cite this paper

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Sabers, C. J., Martin, M. M., Brunn, G. J., Williams, J. M., Dumont, F. J., Wiederrecht, G., & Abraham, R. T. (1995). Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells. The Journal of biological chemistry. https://doi.org/10.1074/jbc.270.2.815

@article{SAB1995,
  author       = {Sabers, C. J. and Martin, M. M. and Brunn, G. J. and Williams, J. M. and Dumont, F. J. and Wiederrecht, G. and Abraham, R. T.},
  title        = {{Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells}},
  journal      = {The Journal of biological chemistry},
  year         = {1995},
  doi          = {10.1074/jbc.270.2.815},
  note         = {PMID: 7822316},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record SAB1995) [Data set]. https://mtor-atlas.org/study/SAB1995/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_SAB1995,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record SAB1995},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/SAB1995/},
  doi          = {10.5281/zenodo.22059963}
}

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