Oliver's mTOR Atlas Evidence Platform
Reading level
Mode

Rapamycin-mediated lifespan increase in mice is dose and sex dependent and metabolically distinct from dietary restriction

Miller RA, Strong R et al. · 2014 · Aging cell · Atlas ID MIL2014

What this study shows

Rapamycin's lifespan extension in mice is dose-dependent and greater in females.

Abstract

ShowHide

Rapamycin, an inhibitor of mTOR kinase, increased median lifespan of genetically heterogeneous mice by 23% (males) to 26% (females) when tested at a dose threefold higher than that used in our previous studies; maximal longevity was also increased in both sexes. Rapamycin increased lifespan more in females than in males at each dose evaluated, perhaps reflecting sexual dimorphism in blood levels of this drug.

Read the full abstract on PubMed →

At a glance

Evidence type A Animal model Marked A because it is an animal intervention or observation study measuring an organismal outcome (model: Mouse); the code names the system studied -- animal work can be rigorous and still not be human data.
Study type4 - Animal Study
Model systemMouse
JournalAging cell
Year2014
Peer reviewedYes
Record last updated2026-08-22
SourceDOI 10.1111/acel.12194 · PMID 24341993 · Free full text (PMC4032600)

Extracted findings

InterventionRapamycin (dose-response)
TargetmTOR
ModelMouse (genetically heterogeneous)
EffectRapamycin extends lifespan 23-26% in a dose- and sex-dependent way, metabolically distinct from dietary restriction
DoseRapamycin in food at 4.7, 14, or 42 ppm, administered from 9 months of age until death.
Sample sizePooled data from 3 sites: 300 control males, 156 males per rapamycin dose group; 280 control females, 136 females per rapamycin dose group.
Effect sizeHighest dose (42 ppm) increased median lifespan by 23% in males (P<0.0001) and 26% in females (P<0.0001); all three doses extended lifespan in females, and the two highest doses extended lifespan in males.
LimitationsLower statistical power for site-independent analyses; Gompertz parameter estimates should be interpreted with caution due to small data sets, low statistical power, and no adjustment for site-to-site variation; increased fighting (11-22% cages removed) in highest dose male group with unknown cause.

In the Atlas

Related topics

mTOR

Open questions that cite this study

Cite this paper

ShowHide 
Miller, R. A., et al. (2014). Rapamycin-mediated lifespan increase in mice is dose and sex dependent and metabolically distinct from dietary restriction. Aging cell. https://doi.org/10.1111/acel.12194

@article{MIL2014,
  author       = {Miller, R. A. and Strong, R. and others},
  title        = {{Rapamycin-mediated lifespan increase in mice is dose and sex dependent and metabolically distinct from dietary restriction}},
  journal      = {Aging cell},
  year         = {2014},
  doi          = {10.1111/acel.12194},
  note         = {PMID: 24341993},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper. 

Barton, O. (2026). Oliver's mTOR Atlas (record MIL2014) [Data set]. https://mtor-atlas.org/study/MIL2014/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_MIL2014,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record MIL2014},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/MIL2014/},
  doi          = {10.5281/zenodo.22059963}
}