Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases

Chung J, Kuo CJ, Crabtree GR, Blenis J · 1992 · Cell · Atlas ID CHU1992

What this study shows

Rapamycin-FKBP12 complex blocks growth-factor activation of p70 S6 kinase, linking the drug to a specific mitogenic pathway.

Abstract

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The macrolide rapamycin blocks cell cycle progression in yeast and various animal cells by an unknown mechanism. We demonstrate that rapamycin blocks the phosphorylation and activation of the 70 kd S6 protein kinases (pp70S6K) in a variety of animal cells. The structurally related drug FK506 had no effect on pp70S6K activation but at high concentrations reversed the rapamycin-induced block, confirming the requirement for the rapamycin and FK506 receptor, FKBP.

Read the full abstract on PubMed →

At a glance

Evidence type	M Molecular — cells, biochemistry, structure Marked M because it is molecular or in-vitro work (model: T cells; in vitro) rather than a whole-organism health-outcome study. That is often exactly where causal biology gets established -- the code says which system the finding was shown in, and nothing about how good the work is.
Study type	5 - Mechanistic / In Vitro
Model system	T cells; in vitro
Journal	Cell
Year	1992
Peer reviewed	Yes
Record last updated	2026-10-01
Source	DOI 10.1016/0092-8674(92)90643-q · PMID 1377606

Extracted findings

These fields were extracted from the paper with AI assistance and have not been checked against the source. Read them as a pointer into the paper, not as curated claims; where they disagree with the curated summary above, the curated summary stands.

Intervention	Rapamycin (± FK506)
Target	FKBP / p70 S6 kinase
Model	T cells; in vitro
Effect	Rapamycin blocks activation of the p70 S6 kinases; FK506 reverses it (FKBP-dependent)

In the Atlas

Links in the pathway map

Learn the biology

Want to understand the biology behind this study? → Challenge 02: Why rapamycin does not switch mTORC1 off

Cite this paper

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Chung, J., Kuo, C. J., Crabtree, G. R., & Blenis, J. (1992). Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases. Cell. https://doi.org/10.1016/0092-8674(92)90643-q

@article{CHU1992,
  author       = {Chung, J. and Kuo, C. J. and Crabtree, G. R. and Blenis, J.},
  title        = {{Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases}},
  journal      = {Cell},
  year         = {1992},
  doi          = {10.1016/0092-8674(92)90643-q},
  note         = {PMID: 1377606},
}

Cite this Atlas record

The record is the Atlas's own work — the evidence label, the extracted findings and the links. It is cited as part of the dataset, not as the paper.

Barton, O. (2026). Oliver's mTOR Atlas (record CHU1992) [Data set]. https://mtor-atlas.org/study/CHU1992/ · Dataset DOI 10.5281/zenodo.22059963

@misc{atlas_CHU1992,
  author       = {Barton, Oliver},
  title        = {{Oliver's mTOR Atlas}, record CHU1992},
  howpublished = {Data set},
  year         = {2026},
  url          = {https://mtor-atlas.org/study/CHU1992/},
  doi          = {10.5281/zenodo.22059963}
}

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