Hediye Erdjument-Bromage
Ran the mass-spectrometry analyses in Paul Tempst's lab at Memorial Sloan Kettering that identified the unknown proteins in the raptor, mLST8 and rictor purifications, and contributed the same approach to work on how ERK inactivates TSC2
Proteomics & mass spectrometry, NYU Langone Health Mass Spectrometry Core for Neuroscience · previously Memorial Sloan Kettering Cancer Center
Mass Spectrometry Core, NYU Langone (New York, New York, USA) ↗ ORCID0000-0003-0224-3594 ↗
Hediye Erdjument-Bromage is a biochemist whose postdoctoral training introduced her to mass spectrometry and proteomics, a technical specialty she has practiced ever since. For many years she managed the microchemistry and proteomics core laboratory at Memorial Sloan Kettering Cancer Center, before moving to NYU Langone Health, where she is now lab manager of the Mass Spectrometry Core for Neuroscience.
Her core facility's protein-identification work was essential to the biochemical purification papers that discovered raptor and rictor — identifying, from tiny amounts of co-purified material, the specific proteins bound to mTOR that turned out to define its two distinct complexes. That kind of unbiased mass-spectrometry identification underlies much of the molecular parts-list this Atlas documents.
The studies below, gathered in this Atlas, mark her contribution.
Milestones in the Atlas
A selection, not a full bibliography: only papers that meet the Atlas inclusion criteria.
| Year | Evidence | Study |
|---|---|---|
| 1994 | M | RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs SAB1994 Discovery of the protein RAFT1 (today's mTOR) as the direct target of the FKBP12-rapamycin complex; one of three 1994 reports (with Brown et al. and Chiu et al.) that identified mammalian TOR, three years after TOR was found in yeast. |
| 2002 | M | mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery KIM2002 Mass-spectrometry identification of raptor, the defining subunit of mTORC1. |
| 2003 | M | GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR KIM2003 Discovered mLST8 (GbetaL), the third core subunit that clamps onto mTOR's kinase domain and stabilizes the complex. It fine-tunes how tightly Raptor holds mTOR in response to nutrients - a small but essential cog that later turned out to be especially critical for the mTORC2 complex. |
| 2004 | M | Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton SAR2004 Mass-spectrometry identification of rictor, the defining subunit of mTORC2. |
| 2005 | M | Phosphorylation and functional inactivation of TSC2 by Erk implications for tuberous sclerosis and cancer pathogenesis MA2005 Contributes proteomics analysis to the identification of TSC2 phosphorylation by Erk. |
Co-authors in the Atlas 8
People with a profile here who share at least one study with Hediye Erdjument-Bromage.
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- Paul Tempst 5 shared studies Developed mass-spectrometry methods for identifying proteins from tiny samples, and led the Memorial Sloan Kettering proteomics laboratory that identified the unknown partners of mTOR in the raptor, mLST8 and rictor purifications
- David M. Sabatini 4 shared studies Identified mTOR (as RAFT1) in 1994. His lab then mapped much of the machinery around it: raptor and rictor at the core of mTORC1 and mTORC2, the Rag–Ragulator system that docks mTORC1 on the lysosome, and the sensors that detect leucine, arginine and SAM
- Do-Hyung Kim 3 shared studies Co-discovered raptor, the defining partner of mTOR in mTORC1, and then mLST8 (GbetaL) as a Sabatini-lab postdoc. His Minnesota lab identified PRAS40 as an insulin-regulated link to mTORC1 and studies how mTORC1 controls autophagy
- Dos D. Sarbassov 3 shared studies Discovered rictor as a Sabatini-lab postdoc, in parallel with Michael Hall's lab, defining mTOR Complex 2, and showed that this complex is the long-sought kinase that phosphorylates Akt on serine 473. He also found that prolonged rapamycin treatment can block mTORC2 assembly
- Robert R. Latek 3 shared studies Bioinformatics scientist at the Whitehead Institute who worked on the Sabatini-lab papers that described raptor, mLST8 (GbetaL) and rictor (raptor and rictor were reported in parallel by other labs), the core partners of mTOR in its two complexes
- Siraj M. Ali 3 shared studies Worked in the Sabatini lab on the papers that discovered raptor, mLST8 and rictor, and on the finding that the rictor-mTOR complex phosphorylates Akt. He later moved into cancer genomics and clinical diagnostics
- David A. Guertin Built the mouse genetics that defined mTORC2's biological roles
- Pier Paolo Pandolfi Showed that ERK directly phosphorylates and inactivates TSC2, wiring Ras/MAPK signalling into mTORC1 control, and that blocking mTORC1 in patients' tumours triggers a feedback rise in MAPK signalling
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